Postdoctoral Position: Molecular Genetic Mechanisms of Male Killing in the Spiroplasma-Drosophila Symbiosis
Indiana University Academic Positions
On-site
Bloomington, Indiana, United States
Position Summary
A postdoctoral position is available in the Newton laboratory (https://biology.indiana.edu/about/faculty/newton-irene-garcia.html), Department of Biology, Indiana University, Bloomington. Our lab investigates mechanisms of symbiosis across insect-microbe systems and uses a combination of genomic/bioinformatic approaches and microbiological, cell biological, genetic and developmental approaches. This position is focused on the bacterial symbiont Spiroplasma poulsonii and characterization of the toxin used by the bacterium to kill male offspring in Drosophila melanogaster. Spiroplasma secretes an androcidin (known as Spaid) that causes this dramatic phenotype. Remarkably, Spaid distinguishes between male and female hosts during infection, killing 99-100% of male offspring while leaving females unharmed. Evidence suggests that Spaid acts through the sex determination pathway to 1) recognize male from female host and 2) induce widespread apoptosis in male but not female embryos. How Spaid carries out these molecular functions is unclear. It is also unclear if D. melanogaster host populations have evolved resistance to Spaid toxicity. Understanding which host proteins Spaid interacts with during infection, whether polymorphisms within these proteins confer toxin resistance, and how modification of these proteins causes male killing will illustrate how a naturally occurring host-parasite interaction unfolds at the molecular level. This project will involve characterizing the molecular targets of Spaid and identifying genetic suppressors in the natural host. A Ph.D. in microbiology, molecular biology, genetics, or a related field is required. Experience with Drosophila melanogaster genetics is preferred. The position will be funded for two years, with the potential to extend for an additional year. Salary will be commensurate with experience, and full benefits are included. To apply, please submit (i) a letter of application, (ii) a full CV, (iii) a statement of research interests, and (iv) contact information for three references electronically to https://indiana.peopleadmin.com/postings/13014 . . For best consideration, please apply by June 30, 2022. Expected start date can be as soon as August 1, 2022 but is flexible. Inquiries about the position can be directed to Irene Newton (irnewton@indiana.edu). Bloomington is a vibrant college town located in scenic southern Indiana, close to several natural parks and wilderness areas, and it enjoys a local culture exceptionally rich in music, art, and theater. Indiana University is an equal employment and affirmative action employer and a provider of ADA services. All qualified applicants will receive consideration for employment based on individual qualifications. Indiana University prohibits discrimination based on age, ethnicity, color, race, religion, sex, sexual orientation, gender identity or expression, genetic information, marital status, national origin, disability status or protected veteran status.
A postdoctoral position is available in the Newton laboratory (https://biology.indiana.edu/about/faculty/newton-irene-garcia.html), Department of Biology, Indiana University, Bloomington. Our lab investigates mechanisms of symbiosis across insect-microbe systems and uses a combination of genomic/bioinformatic approaches and microbiological, cell biological, genetic and developmental approaches. This position is focused on the bacterial symbiont Spiroplasma poulsonii and characterization of the toxin used by the bacterium to kill male offspring in Drosophila melanogaster. Spiroplasma secretes an androcidin (known as Spaid) that causes this dramatic phenotype. Remarkably, Spaid distinguishes between male and female hosts during infection, killing 99-100% of male offspring while leaving females unharmed. Evidence suggests that Spaid acts through the sex determination pathway to 1) recognize male from female host and 2) induce widespread apoptosis in male but not female embryos. How Spaid carries out these molecular functions is unclear. It is also unclear if D. melanogaster host populations have evolved resistance to Spaid toxicity. Understanding which host proteins Spaid interacts with during infection, whether polymorphisms within these proteins confer toxin resistance, and how modification of these proteins causes male killing will illustrate how a naturally occurring host-parasite interaction unfolds at the molecular level. This project will involve characterizing the molecular targets of Spaid and identifying genetic suppressors in the natural host. A Ph.D. in microbiology, molecular biology, genetics, or a related field is required. Experience with Drosophila melanogaster genetics is preferred. The position will be funded for two years, with the potential to extend for an additional year. Salary will be commensurate with experience, and full benefits are included. To apply, please submit (i) a letter of application, (ii) a full CV, (iii) a statement of research interests, and (iv) contact information for three references electronically to https://indiana.peopleadmin.com/postings/13014 . . For best consideration, please apply by June 30, 2022. Expected start date can be as soon as August 1, 2022 but is flexible. Inquiries about the position can be directed to Irene Newton (irnewton@indiana.edu). Bloomington is a vibrant college town located in scenic southern Indiana, close to several natural parks and wilderness areas, and it enjoys a local culture exceptionally rich in music, art, and theater. Indiana University is an equal employment and affirmative action employer and a provider of ADA services. All qualified applicants will receive consideration for employment based on individual qualifications. Indiana University prohibits discrimination based on age, ethnicity, color, race, religion, sex, sexual orientation, gender identity or expression, genetic information, marital status, national origin, disability status or protected veteran status.